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PDBsum entry 1bd2
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Complex (mhc/viral peptide/receptor)
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PDB id
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1bd2
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Contents |
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275 a.a.
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99 a.a.
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190 a.a.
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242 a.a.
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Two human t cell receptors bind in a similar diagonal mode to the hla-A2/tax peptide complex using different tcr amino acids.
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Authors
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Y.H.Ding,
K.J.Smith,
D.N.Garboczi,
U.Utz,
W.E.Biddison,
D.C.Wiley.
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Ref.
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Immunity, 1998,
8,
403-411.
[DOI no: ]
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PubMed id
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Abstract
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The three-dimensional structure of a human alphabeta T cell receptor (TCR), B7,
bound to the HLA-A2 molecule/HTLV-1 Tax peptide complex was determined by x-ray
crystallography. Although different from the A6 TCR, previously studied, in 16
of the 17 residues that contact HLA-A2/Tax, the B7 TCR binds in a similar
diagonal manner, only slightly tipped and rotated, relative to the A6 TCR. The
structure explains data from functional assays on the specificity differences
between the B7 and A6 TCRs for agonist, partial agonist, and null peptides. The
existence of a structurally similar diagonal binding mode for TCRs favors
mechanisms based on the formation of geometrically defined supramolecular
assemblies for initiating signaling.
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Figure 1.
Figure 1. Comparison of the Diagonal Binding Modes of the
Human B7 TCR and A6 TCR Bound to the HLA-A2/HTLV-1 Tax Peptide
Complex(A) Shown are the three variable or CDR loops of B7 TCR
Vα (red) and Vβ (yellow); A6 TCR (blue); HLA (dark blue); and
peptide (white). The variable loop 4 of Vα is omitted because
it makes no contacts in the B7 TCR complex.(B) The Vα and Vβ
domains of each TCR bound to HLA-A2 (colors as in [A]). The
α[1]α[2] domains of HLA-A2 from each TCR/MHC structure are
superimposed.(C) TCR/HLA-A2/Tax complexes are compared by
superimposing the HLA-A2 (blue) molecule. B7 TCR (red); A6 TCR
(green). B7–CDR2β contacts HLA-A2 (arrow); A6–CDR2β does
not.(D) Vα (blue)–Vβ (red) interface in B7 and A6 (light
colors). β chains of B7 and A6 are superimposed.Figure drawn
with RIBBONS ([6]).
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Figure 3.
Figure 3. The Two TCR Surfaces That Contact the Same
MHC/Peptide Complex(A) The molecular surface of the B7 TCR with
a ball-and-stick model of the bound Tax peptide superimposed.
Surface color: red, negative electrostatic charge potential;
blue, positive.(B) The molecular surface of the A6 TCR with a
ball-and-stick model of the bound Tax peptide
superimposed.Figure drawn with GRASP ([28]).
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The above figures are
reprinted
by permission from Cell Press:
Immunity
(1998,
8,
403-411)
copyright 1998.
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Secondary reference #1
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Title
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Structure of the complex between human t-Cell receptor, Viral peptide and hla-A2.
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Authors
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D.N.Garboczi,
P.Ghosh,
U.Utz,
Q.R.Fan,
W.E.Biddison,
D.C.Wiley.
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Ref.
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Nature, 1996,
384,
134-141.
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PubMed id
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