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PDBsum entry 1b9g
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Growth factor
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PDB id
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1b9g
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Solution structure of a mini igf-1.
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Authors
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E.De wolf,
R.Gill,
S.Geddes,
J.Pitts,
A.Wollmer,
J.Grötzinger.
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Ref.
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Protein Sci, 1996,
5,
2193-2202.
[DOI no: ]
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PubMed id
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Abstract
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Mini insulin-like growth factor 1, an inactive insulin-like growth factor 1
mutant lacking the C region, was studied by 2D NMR spectroscopy. Resonances were
assigned for almost all protons of the 57 amino acid residues. The 3D structure
of the protein was determined by distance geometry methods. Three helical
segments; Ala 8-Cys 18, Gly 42-Phe 49, and Leu 54-Cys 61, were identified,
corresponding to those present in wild-type insulin-like growth factor 1 and in
single-chain insulin. Their relative orientation, however, was found to be
changed. This change is connected with a displacement of the Phe 23-Tyr 24-Phe
25-Asn 26 beta-strand-like segment, i.e., of aromatic side chains known to be
important for receptor binding. Thus, deletion of the C region of IGF-1 results
in a substantial tertiary structural rearrangement that accounts for the loss of
receptor affinity.
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