UniProt functional annotation for P06181



	UniProt functional annotation for P06181

UniProt code: P06181.

Organism: Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum).

Taxonomy: Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Polyporales; Phanerochaetaceae; Phanerochaete.

Function: Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin. {ECO:0000269|PubMed:16593451, ECO:0000269|PubMed:2303054}.

Catalytic activity: Reaction=1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + glycolaldehyde + guaiacol + H2O; Xref=Rhea:RHEA:48004, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17071, ChEBI:CHEBI:17098, ChEBI:CHEBI:28591, ChEBI:CHEBI:86963; EC=1.11.1.14; Evidence={ECO:0000269|PubMed:16593451, ECO:0000269|PubMed:2303054};

Catalytic activity: Reaction=2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4- dimethoxyphenyl)methanol radical + 2 H2O; Xref=Rhea:RHEA:30271, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:62150, ChEBI:CHEBI:88143; EC=1.11.1.14; Evidence={ECO:0000269|PubMed:16593451, ECO:0000269|PubMed:2303054};

Cofactor: Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 2 calcium ions per subunit.;

Cofactor: Name=heme b; Xref=ChEBI:CHEBI:60344; Evidence={ECO:0000269|PubMed:16593451}; Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit. {ECO:0000269|PubMed:16593451};

Biophysicochemical properties: Kinetic parameters: KM=130 uM for H(2)O(2) {ECO:0000269|PubMed:2303054}; KM=116 uM for (3,4-dimethoxyphenyl)methanol {ECO:0000269|PubMed:3240864}; KM=30 uM for H(2)O(2) {ECO:0000269|PubMed:16593451}; pH dependence: Optimum pH is 3. {ECO:0000269|PubMed:16593451, ECO:0000269|PubMed:2303054};

Pathway: Secondary metabolite metabolism; lignin degradation.

Developmental stage: Ligninases are expressed during secondary metabolism, and are triggered by nutrient limitation.

Similarity: Belongs to the peroxidase family. Ligninase subfamily. {ECO:0000305}.

Sequence caution: Sequence=AAA33740.1; Type=Erroneous translation; Evidence={ECO:0000305}; Sequence=CAA68373.1; Type=Frameshift; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum).
Taxonomy:		Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Polyporales; Phanerochaetaceae; Phanerochaete.



Function:		Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin. {ECO:0000269\|PubMed:16593451, ECO:0000269\|PubMed:2303054}.


Catalytic activity:		Reaction=1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + glycolaldehyde + guaiacol + H2O; Xref=Rhea:RHEA:48004, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:17071, ChEBI:CHEBI:17098, ChEBI:CHEBI:28591, ChEBI:CHEBI:86963; EC=1.11.1.14; Evidence={ECO:0000269\|PubMed:16593451, ECO:0000269\|PubMed:2303054};
Catalytic activity:		Reaction=2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4- dimethoxyphenyl)methanol radical + 2 H2O; Xref=Rhea:RHEA:30271, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:62150, ChEBI:CHEBI:88143; EC=1.11.1.14; Evidence={ECO:0000269\|PubMed:16593451, ECO:0000269\|PubMed:2303054};
Cofactor:		Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 2 calcium ions per subunit.;
Cofactor:		Name=heme b; Xref=ChEBI:CHEBI:60344; Evidence={ECO:0000269\|PubMed:16593451}; Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit. {ECO:0000269\|PubMed:16593451};
Biophysicochemical properties:		Kinetic parameters: KM=130 uM for H(2)O(2) {ECO:0000269\|PubMed:2303054}; KM=116 uM for (3,4-dimethoxyphenyl)methanol {ECO:0000269\|PubMed:3240864}; KM=30 uM for H(2)O(2) {ECO:0000269\|PubMed:16593451}; pH dependence: Optimum pH is 3. {ECO:0000269\|PubMed:16593451, ECO:0000269\|PubMed:2303054};
Pathway:		Secondary metabolite metabolism; lignin degradation.
Developmental stage:		Ligninases are expressed during secondary metabolism, and are triggered by nutrient limitation.
Similarity:		Belongs to the peroxidase family. Ligninase subfamily. {ECO:0000305}.
Sequence caution:		Sequence=AAA33740.1; Type=Erroneous translation; Evidence={ECO:0000305}; Sequence=CAA68373.1; Type=Frameshift; Evidence={ECO:0000305};