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PDBsum entry 1axh
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References listed in PDB file
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Key reference
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Title
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The structure of a novel insecticidal neurotoxin, Omega-Atracotoxin-Hv1, From the venom of an australian funnel web spider.
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Authors
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J.I.Fletcher,
R.Smith,
S.I.O'Donoghue,
M.Nilges,
M.Connor,
M.E.Howden,
M.J.Christie,
G.F.King.
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Ref.
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Nat Struct Biol, 1997,
4,
559-566.
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PubMed id
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Abstract
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A family of potent insecticidal toxins has recently been isolated from the venom
of Australian funnel web spiders. Among these is the 37-residue peptide
omega-atracotoxin-HV1 (omega-ACTX-HV1) from Hadronyche versuta. We have
chemically synthesized and folded omega-ACTX-HV1, shown that it is neurotoxic,
ascertained its disulphide bonding pattern, and determined its three-dimensional
solution structure using NMR spectroscopy. The structure consists of a
solvent-accessible beta-hairpin protruding from a disulphide-bonded globular
core comprising four beta-turns. The three intramolecular disulphide bonds from
a cystine knot motif similar to that seen in several other neurotoxic peptides.
Despite limited sequence identity, omega-ACTX-HV1 displays significant
structural homology with the omega-agatoxins and omega-conotoxins, both of which
are vertebrate calcium channel antagonists; however, in contrast with these
toxins, we show that omega-ACTX-HV1 inhibits insect, but not mammalian,
voltage-gated calcium channel currents.
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Secondary reference #1
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Title
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Authors
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R.K.Atkinson,
M.I.Tyler,
E.J.Vonarx,
M.E.H.Howden.
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Ref.
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insecticidal toxins derived ...
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