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UniProt functional annotation for P80293 | ||
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| UniProt code: P80293. |
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| Organism: | |
Propionibacterium freudenreichii subsp. shermanii. |
| Taxonomy: | |
Bacteria; Actinobacteria; Propionibacteriales; Propionibacteriaceae; Propionibacterium. |
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| Function: | |
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the dismutation of superoxide anion radicals into O2 and H2O2 by successive reduction and oxidation of the transition metal ion at the active site. {ECO:0000269|PubMed:7575431}. |
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| Catalytic activity: | |
Reaction=2 H(+) + 2 superoxide = H2O2 + O2; Xref=Rhea:RHEA:20696, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:18421; EC=1.15.1.1; Evidence={ECO:0000269|PubMed:7575431}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20697; Evidence={ECO:0000305|PubMed:7575431}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:7575431, ECO:0000269|PubMed:9531477, ECO:0000269|Ref.4}; Name=Fe(3+); Xref=ChEBI:CHEBI:29034; Evidence={ECO:0000269|PubMed:10231372, ECO:0000269|PubMed:7575431, ECO:0000269|PubMed:9531477, ECO:0000269|Ref.4}; Note=Binds 1 Mn(2+) or Fe(3+) ion per subunit. {ECO:0000269|PubMed:10231372, ECO:0000269|Ref.4}; |
| Activity regulation: | |
Shows decreasing activity with increasing pH (PubMed:7575431). Slightly inhibited by azide and fluoride at pH 7-8; the inhibition is drastically increased towards lower pH (PubMed:9531477). {ECO:0000269|PubMed:7575431, ECO:0000269|PubMed:9531477}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.54 mM for superoxide (at pH 9.5, with the Fe-SOD form) {ECO:0000269|PubMed:7575431}; |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:8307013}. |
| Similarity: | |
Belongs to the iron/manganese superoxide dismutase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.