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PDBsum entry 1aif
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Immunoglobulin
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PDB id
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1aif
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of an anti-Idiotypic FAB against feline peritonitis virus-Neutralizing antibody and a comparison with the complexed FAB.
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Authors
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N.Ban,
C.Escobar,
K.W.Hasel,
J.Day,
A.Greenwood,
A.Mcpherson.
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Ref.
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Faseb J, 1995,
9,
107-114.
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PubMed id
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Abstract
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The crystal structure of anti-idiotopic Fab 409.5.3, made against an E2 specific
feline infectious peritonitis virus-neutralizing antibody 730.1.4, has been
determined in its free from, at 2.9 A resolution by molecular replacement. This
antibody, used as an immmunogen, elicits the production of anti-anti-idiotypic
antibodies that in turn neutralize the virus. The structure of the uncomplexed
Fab was refined using constrained-restrained least squares minimization and
simulated annealing in combination with conjugate gradient techniques to a
crystallographic R of 0.22 based on 16,482 unique reflections between 20.0 and
2.9 A. The free antiidiotypic Fab shows, when compared to its complexed form, a
5 degrees rotation of its variable light with respect to its variable heavy
domain and rearrangement of complementarity determining region loops, which
permits optimization of the stereocomplementarity between interacting molecules.
This finding supports the induced fit hypothesis for antibody antigen
interaction.
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Secondary reference #1
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Title
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Crystal structure of an idiotype-Anti-Idiotype FAB complex.
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Authors
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N.Ban,
C.Escobar,
R.Garcia,
K.Hasel,
J.Day,
A.Greenwood,
A.Mcpherson.
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Ref.
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Proc Natl Acad Sci U S A, 1994,
91,
1604-1608.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Preliminary crystallographic study of a complex between an FAB of a monoclonal feline peritonitis virus neutralizing antibody and its anti-Idiotypic FAB.
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Authors
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N.Ban,
C.Escobar,
J.Day,
A.Greenwood,
A.Mcpherson.
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Ref.
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J Mol Biol, 1993,
234,
894-896.
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PubMed id
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Secondary reference #3
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Title
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Characterization of crystals of an FAB fragment of a murine monoclonal antibody.
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Authors
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N.Ban,
C.Escobar,
J.Day,
A.Greenwood,
S.Larson,
A.Mcpherson.
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Ref.
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J Mol Biol, 1991,
222,
445-446.
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PubMed id
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