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PDBsum entry 1af5
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References listed in PDB file
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Key reference
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Title
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The structure of I-Crel, A group I intron-Encoded homing endonuclease.
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Authors
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P.J.Heath,
K.M.Stephens,
R.J.Monnat,
B.L.Stoddard.
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Ref.
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Nat Struct Biol, 1997,
4,
468-476.
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PubMed id
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Abstract
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The structure of I-Crel provides the first view of a protein encoded by a gene
within an intron. This endonuclease recognizes a long DNA site approximately 20
base pairs in length and facilitates the lateral transfer of that intron. The
protein exhibits a DNA-binding surface consisting of four antiparallel
beta-strands that form a 20 A wide groove which is over 70 A long. The
architecture of this fold is different from that of the TATA binding protein,
TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG
motif, which is found in many mobile intron endonucleases, maturases and
inteins, forms a novel helical interface and contributes essential residues to
the active site.
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