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UniProt functional annotation for P50578 | ||
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| UniProt code: P50578. |
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| Organism: | |
Sus scrofa (Pig). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus. |
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| Function: | |
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde (By similarity). Plays an important role in ascorbic acid biosynthesis by catalyzing the reduction of D-glucuronic acid and D-glucurono-gamma-lactone (By similarity). Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs (By similarity). Displays no reductase activity towards retinoids (PubMed:12732097). {ECO:0000250|UniProtKB:P14550, ECO:0000250|UniProtKB:P51635, ECO:0000269|PubMed:12732097}. |
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| Catalytic activity: | |
Reaction=a primary alcohol + NADP(+) = an aldehyde + H(+) + NADPH; Xref=Rhea:RHEA:15937, ChEBI:CHEBI:15378, ChEBI:CHEBI:15734, ChEBI:CHEBI:17478, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.2; Evidence={ECO:0000269|PubMed:12732097}; |
| Catalytic activity: | |
Reaction=glycerol + NADP(+) = D-glyceraldehyde + H(+) + NADPH; Xref=Rhea:RHEA:23592, ChEBI:CHEBI:15378, ChEBI:CHEBI:17378, ChEBI:CHEBI:17754, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.372; Evidence={ECO:0000269|PubMed:12732097}; |
| Catalytic activity: | |
Reaction=glycerol + NADP(+) = H(+) + L-glyceraldehyde + NADPH; Xref=Rhea:RHEA:38111, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:27975, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.372; Evidence={ECO:0000269|PubMed:12732097}; |
| Catalytic activity: | |
Reaction=L-gulonate + NADP(+) = aldehydo-D-glucuronate + H(+) + NADPH; Xref=Rhea:RHEA:14909, ChEBI:CHEBI:13115, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:142686; EC=1.1.1.19; Evidence={ECO:0000250|UniProtKB:P51635}; |
| Catalytic activity: | |
Reaction=L-gulono-1,4-lactone + NADP(+) = D-glucurono-3,6-lactone + H(+) + NADPH; Xref=Rhea:RHEA:18925, ChEBI:CHEBI:15378, ChEBI:CHEBI:17587, ChEBI:CHEBI:18268, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.20; Evidence={ECO:0000250|UniProtKB:P51635}; |
| Catalytic activity: | |
Reaction=allyl alcohol + NADP(+) = acrolein + H(+) + NADPH; Xref=Rhea:RHEA:12168, ChEBI:CHEBI:15368, ChEBI:CHEBI:15378, ChEBI:CHEBI:16605, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.54; Evidence={ECO:0000250|UniProtKB:P51635}; |
| Catalytic activity: | |
Reaction=hydroxyacetone + NADP(+) = H(+) + methylglyoxal + NADPH; Xref=Rhea:RHEA:27986, ChEBI:CHEBI:15378, ChEBI:CHEBI:17158, ChEBI:CHEBI:27957, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000250|UniProtKB:P51635}; |
| Catalytic activity: | |
Reaction=3-deoxyfructose + NADP(+) = 3-deoxyglucosone + H(+) + NADPH; Xref=Rhea:RHEA:58668, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:60777, ChEBI:CHEBI:142685; Evidence={ECO:0000250|UniProtKB:P51635}; |
| Catalytic activity: | |
Reaction=(R)-mevalonate + NADP(+) = H(+) + mevaldate + NADPH; Xref=Rhea:RHEA:20193, ChEBI:CHEBI:15378, ChEBI:CHEBI:36464, ChEBI:CHEBI:57783, ChEBI:CHEBI:58219, ChEBI:CHEBI:58349; EC=1.1.1.33; Evidence={ECO:0000250|UniProtKB:P51635}; |
| Catalytic activity: | |
Reaction=NADP(+) + pyridine 3-methanol = H(+) + NADPH + pyridine-3- carbaldehyde; Xref=Rhea:RHEA:58776, ChEBI:CHEBI:15378, ChEBI:CHEBI:28345, ChEBI:CHEBI:45213, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:12732097}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=700 uM for pyridine-3-carbaldehyde {ECO:0000269|PubMed:12732097}; KM=4600 uM for D,L-glyceraldehyde {ECO:0000269|PubMed:12732097}; Note=kcat is 11000 min(-1) for pyridine-3-carbaldehyde as substrate. kcat is 2500 min(-1) for D,L-glyceraldehyde as substrate. {ECO:0000269|PubMed:12732097}; |
| Subunit: | |
Monomer. |
| Subcellular location: | |
Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9JII6}. Apical cell membrane {ECO:0000250|UniProtKB:Q9JII6}. |
| Similarity: | |
Belongs to the aldo/keto reductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.