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PDBsum entry 1add
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Hydrolase(acting in cyclicamidines)
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PDB id
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1add
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References listed in PDB file
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Key reference
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Title
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A pre-Transition-State mimic of an enzyme: X-Ray structure of adenosine deaminase with bound 1-Deazaadenosine and zinc-Activated water.
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Authors
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D.K.Wilson,
F.A.Quiocho.
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Ref.
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Biochemistry, 1993,
32,
1689-1694.
[DOI no: ]
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PubMed id
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Abstract
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The refined 2.4-A structure of adenosine deaminase, recently discovered to be a
zinc metalloenzyme [Wilson, D. K., Rudolph, F. B., & Quiocho, F. A. (1991)
Science 252, 1278-1284], complexed with the ground-state analog 1-deazaadenosine
shows the mode of binding of the analog and, unexpectedly, a zinc-activated
water (hydroxide). This structure of a pre-transition-state mimic, combined with
that previously determined for the complex with 6(R)-hydroxy-1,6-dihydropurine
ribonucleoside, a nearly ideal transition-state analog, sheds new understanding
of the precise stereospecificity and hydrolytic catalysis of an important and
well-characterized member of a large group of zinc metalloenzymes. As both of
these excellent mimics were generated in the active site, they demonstrate a
powerful means of dissecting the course of an enzymatic reaction by direct
crystallographic analysis.
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Secondary reference #1
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Title
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Atomic structure of adenosine deaminase complexed with a transition-State analog: understanding catalysis and immunodeficiency mutations.
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Authors
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D.K.Wilson,
F.B.Rudolph,
F.A.Quiocho.
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Ref.
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Science, 1991,
252,
1278-1284.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Preliminary X-Ray analysis of crystals of murine adenosine deaminase.
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Authors
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D.K.Wilson,
F.B.Rudolph,
M.L.Harrison,
R.E.Kellems,
F.A.Quiocho.
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Ref.
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J Mol Biol, 1988,
200,
613-614.
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PubMed id
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