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PDBsum entry 1aal
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Serine protease inhibitor
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PDB id
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1aal
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structural effects induced by mutagenesis affected by crystal packing factors: the structure of a 30-51 disulfide mutant of basic pancreatic trypsin inhibitor.
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Authors
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C.Eigenbrot,
M.Randal,
A.A.Kossiakoff.
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Ref.
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Proteins, 1992,
14,
75-87.
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PubMed id
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Abstract
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The X-ray structure of the C30V/C51A disulfide mutant of basic pancreatic
trypsin inhibitor (BPTI) has been analyzed at 1.6 A resolution. The mutant
crystallizes in a cell having two molecules in the asymmetric unit. The packing
environments of these two molecules are quite different, allowing for an
assessment of which among the observed structural changes result from the
mutation and which are produced by lattice packing considerations. The removal
of the 30-51 disulfide bridge has little apparent affect on the B-factors of
segments of adjacent polypeptide chain, although there are distinct differences
in the structure compared to wild-type BPTI crystal structures. Both of the two
C30V/C51A molecules show differences at the mutation site when compared to
another 30-51 disulfide mutant, C30A/C51A, presumably due to the larger steric
bulk of a valine versus an alanine at residue 30. A comparison of the two
independent C30V/C51A molecules indicates that there are significant differences
between them even at the site of mutation. The description of the specific
structural differences of each molecule differs in detail and suggests different
conclusions about the nature of structural perturbation near 30-51. In addition,
when these two molecules are compared to two different wild-type structures,
which had been determined from different space groups, a somewhat different
pattern of changes is observed. These findings indicate that crystal packing can
influence the observed perturbations in mutant structures.
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Secondary reference #1
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Title
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Structural effects induced by removal of a disulfide-Bridge: the X-Ray structure of the c30a/c51a mutant of basic pancreatic trypsin inhibitor at 1.6 a.
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Authors
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C.Eigenbrot,
M.Randal,
A.A.Kossiakoff.
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Ref.
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Protein Eng, 1990,
3,
591-598.
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PubMed id
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