PDBsum entry 1a8d

Neurotoxin

PDB id: 1a8d

Contents

Protein chain

452 a.a. *

Metals

_AU ×2

Waters ×424

* Residue conservation analysis

PDB id:

1a8d

Name:

Neurotoxin

Title:

Tetanus toxin c fragment

Structure:

Tetanus neurotoxin. Chain: a. Fragment: c-fragment. Engineered: yes. Mutation: yes. Other_details: gold (au) derivative

Source:

Clostridium tetani. Organism_taxid: 1513. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: recombinant protein, purchased from boehringer- mannheim catalog number 1348-655

Resolution:

1.57Å R-factor: 0.187 R-free: 0.262

Authors:

M.Knapp,B.Rupp

Key ref:

M.Knapp et al. (1998). The 1.61 angstrom structure of the tetanus toxin ganglioside binding region: solved by mad and mir phase combination. Am cryst assoc ,Abstr papers (annual meeting), 25, 90.

Date:

23-Mar-98 Release date: 14-Oct-98

Protein chain

P04958  (TETX_CLOTE) -  Tetanus toxin from Clostridium tetani (strain Massachusetts / E88)

Seq: 1315 a.a.

Struc: 452 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.24.68 - tentoxilysin.
• Reaction: Hydrolysis of 76-Gln-|-Phe-77 bond in synaptobrevin 2.
• Cofactor: Zn(2+)