UniProt functional annotation for P18316



	UniProt functional annotation for P18316

UniProt code: P18316.

Organism: Klebsiella aerogenes (Enterobacter aerogenes).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella.

Catalytic activity: Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5; Evidence={ECO:0000255|HAMAP-Rule:MF_00739, ECO:0000269|PubMed:10500143, ECO:0000269|PubMed:11015224, ECO:0000269|PubMed:1400317, ECO:0000269|PubMed:2211515, ECO:0000269|PubMed:7721685, ECO:0000269|PubMed:7855593, ECO:0000269|PubMed:7909161, ECO:0000269|PubMed:8318888, ECO:0000269|PubMed:8808930};

Activity regulation: The apoenzyme can be activated in vitro in the presence of nickel ions and carbon dioxide, which promotes carbamylation of 'Lys-217' of the UreC (alpha) subunit. {ECO:0000269|PubMed:10500143, ECO:0000269|PubMed:11015224, ECO:0000269|PubMed:7855593, ECO:0000269|PubMed:8808930}.

Biophysicochemical properties: Kinetic parameters: KM=2.3 mM for urea {ECO:0000269|PubMed:10913264, ECO:0000269|PubMed:11594743, ECO:0000269|PubMed:1400317, ECO:0000269|PubMed:8318888}; Vmax=1.9 mmol/min/mg enzyme {ECO:0000269|PubMed:10913264, ECO:0000269|PubMed:11594743, ECO:0000269|PubMed:1400317, ECO:0000269|PubMed:8318888}; pH dependence: Optimum pH is 7.75. {ECO:0000269|PubMed:10913264, ECO:0000269|PubMed:11594743, ECO:0000269|PubMed:1400317, ECO:0000269|PubMed:8318888};

Pathway: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00739}.

Subunit: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. The apoenzyme interacts with an accessory complex composed of UreD, UreF and UreG, which is required for the assembly of the nickel containing metallocenter of UreC. The UreE protein may also play a direct role as a metallochaperone in nickel transfer to the urease apoprotein. {ECO:0000255|HAMAP-Rule:MF_00739, ECO:0000269|PubMed:10500143, ECO:0000269|PubMed:10555581, ECO:0000269|PubMed:10913264, ECO:0000269|PubMed:14749331, ECO:0000269|PubMed:7721685, ECO:0000269|PubMed:7754395, ECO:0000269|PubMed:7909161, ECO:0000269|PubMed:8702515, ECO:0000269|PubMed:8718850, ECO:0000269|PubMed:8808930, ECO:0000269|PubMed:9201965, ECO:0000269|PubMed:9558361}.

Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00739}.

Similarity: Belongs to the urease gamma subunit family. {ECO:0000255|HAMAP-Rule:MF_00739}.

Annotations taken from UniProtKB at the EBI.


Organism:		Klebsiella aerogenes (Enterobacter aerogenes).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella.


Catalytic activity:		Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5; Evidence={ECO:0000255\|HAMAP-Rule:MF_00739, ECO:0000269\|PubMed:10500143, ECO:0000269\|PubMed:11015224, ECO:0000269\|PubMed:1400317, ECO:0000269\|PubMed:2211515, ECO:0000269\|PubMed:7721685, ECO:0000269\|PubMed:7855593, ECO:0000269\|PubMed:7909161, ECO:0000269\|PubMed:8318888, ECO:0000269\|PubMed:8808930};
Activity regulation:		The apoenzyme can be activated in vitro in the presence of nickel ions and carbon dioxide, which promotes carbamylation of 'Lys-217' of the UreC (alpha) subunit. {ECO:0000269\|PubMed:10500143, ECO:0000269\|PubMed:11015224, ECO:0000269\|PubMed:7855593, ECO:0000269\|PubMed:8808930}.
Biophysicochemical properties:		Kinetic parameters: KM=2.3 mM for urea {ECO:0000269\|PubMed:10913264, ECO:0000269\|PubMed:11594743, ECO:0000269\|PubMed:1400317, ECO:0000269\|PubMed:8318888}; Vmax=1.9 mmol/min/mg enzyme {ECO:0000269\|PubMed:10913264, ECO:0000269\|PubMed:11594743, ECO:0000269\|PubMed:1400317, ECO:0000269\|PubMed:8318888}; pH dependence: Optimum pH is 7.75. {ECO:0000269\|PubMed:10913264, ECO:0000269\|PubMed:11594743, ECO:0000269\|PubMed:1400317, ECO:0000269\|PubMed:8318888};
Pathway:		Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. {ECO:0000255\|HAMAP-Rule:MF_00739}.
Subunit:		Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme. The apoenzyme interacts with an accessory complex composed of UreD, UreF and UreG, which is required for the assembly of the nickel containing metallocenter of UreC. The UreE protein may also play a direct role as a metallochaperone in nickel transfer to the urease apoprotein. {ECO:0000255\|HAMAP-Rule:MF_00739, ECO:0000269\|PubMed:10500143, ECO:0000269\|PubMed:10555581, ECO:0000269\|PubMed:10913264, ECO:0000269\|PubMed:14749331, ECO:0000269\|PubMed:7721685, ECO:0000269\|PubMed:7754395, ECO:0000269\|PubMed:7909161, ECO:0000269\|PubMed:8702515, ECO:0000269\|PubMed:8718850, ECO:0000269\|PubMed:8808930, ECO:0000269\|PubMed:9201965, ECO:0000269\|PubMed:9558361}.
Subcellular location:		Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_00739}.
Similarity:		Belongs to the urease gamma subunit family. {ECO:0000255\|HAMAP-Rule:MF_00739}.