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UniProt functional annotation for O34002 | ||
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| UniProt code: O34002. |
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| Organism: | |
Antarctic bacterium DS2-3R. |
| Taxonomy: | |
Bacteria. |
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| Function: | |
Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity. {ECO:0000269|PubMed:9310359, ECO:0000269|PubMed:9579066}. |
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| Catalytic activity: | |
Reaction=H2O + oxaloacetate + propanoyl-CoA = (2S,3S)-2-methylcitrate + CoA + H(+); Xref=Rhea:RHEA:23780, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, ChEBI:CHEBI:57287, ChEBI:CHEBI:57392, ChEBI:CHEBI:58853; EC=2.3.3.5; Evidence={ECO:0000269|PubMed:9579066}; |
| Catalytic activity: | |
Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+); Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000269|PubMed:9310359, ECO:0000269|PubMed:9579066}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3 uM for oxaloacetate (with propionyl-CoA at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9579066}; KM=6.9 uM for oxaloacetate (with acetyl-CoA at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9310359}; KM=7 uM for oxaloacetate (with acetyl-CoA at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9579066}; KM=16 uM for propionyl-CoA (at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9579066}; KM=229 uM for acetyl-CoA (at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9579066}; KM=230 uM for acetyl-CoA (at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9310359}; Vmax=12 umol/min/mg enzyme with propionyl-CoA as substrate (at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9579066}; Vmax=30 umol/min/mg enzyme with acetyl-CoA as substrate (at pH 8 and 23 degrees Celsius) {ECO:0000269|PubMed:9310359, ECO:0000269|PubMed:9579066}; Note=Kcat is 8 sec(-1) for 2-methylcitrate synthase activity with propionyl-CoA as substrate (at pH 8 and 23 degrees Celsius). Kcat is 21 sec(-1) for citrate synthase activity with acetyl-CoA as substrate (at pH 8 and 23 degrees Celsius). {ECO:0000269|PubMed:9310359, ECO:0000269|PubMed:9579066}; Temperature dependence: Optimum temperature is 31 degrees Celsius. Cold-active. Is rapidly inactivated at 45 degrees Celsius, and shows significant activity at 10 degrees Celsius and below. {ECO:0000269|PubMed:9310359}; |
| Pathway: | |
Organic acid metabolism; propanoate degradation. {ECO:0000305|PubMed:9579066}. |
| Pathway: | |
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. {ECO:0000305|PubMed:9579066}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:9310359, ECO:0000269|PubMed:9551556}. |
| Induction: | |
By growth on propionate, but not acetate or glucose. {ECO:0000269|PubMed:9579066}. |
| Similarity: | |
Belongs to the citrate synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.