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PDBsum entry 1a54
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Phosphotransferase
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PDB id
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1a54
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, A probe for inorganic phosphate.
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Authors
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M.Hirshberg,
K.Henrick,
L.L.Haire,
N.Vasisht,
M.Brune,
J.E.Corrie,
M.R.Webb.
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Ref.
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Biochemistry, 1998,
37,
10381-10385.
[DOI no: ]
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PubMed id
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Abstract
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Crystal structures are presented for the A197C mutant of Escherichia coli
phosphate binding protein (PBP) and the same mutant labeled at Cys197 with
N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Both
proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP,
exhibits a large increase in fluorescence on binding inorganic phosphate. The
resulting high-fluorescence state of the coumarin and the ability of this
coumarin to monitor the conformational changes associated with inorganic
phosphate binding are interpreted in terms of the specific interactions of MDCC
with the protein. The structure helps to explain why this particular label gives
a high-fluorescence state on binding inorganic phosphate, while several other
related labels do not, and hence aids our general understanding of
environmentally sensitive fluorescence probes on proteins.
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Secondary reference #1
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Title
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Mechanism of inorganic phosphate interaction with phosphate binding protein from escherichia coli.
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Authors
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M.Brune,
J.L.Hunter,
S.A.Howell,
S.R.Martin,
T.L.Hazlett,
J.E.Corrie,
M.R.Webb.
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Ref.
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Biochemistry, 1998,
37,
10370-10380.
[DOI no: ]
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PubMed id
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