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PDBsum entry 1a3k
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References listed in PDB file
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Key reference
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Title
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X-Ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.
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Authors
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J.Seetharaman,
A.Kanigsberg,
R.Slaaby,
H.Leffler,
S.H.Barondes,
J.M.Rini.
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Ref.
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J Biol Chem, 1998,
273,
13047-13052.
[DOI no: ]
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PubMed id
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Abstract
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Galectins are a family of lectins which share similar carbohydrate recognition
domains (CRDs) and affinity for small beta-galactosides, but which show
significant differences in binding specificity for more complex glycoconjugates.
We report here the x-ray crystal structure of the human galectin-3 CRD, in
complex with lactose and N-acetyllactosamine, at 2.1-A resolution. This
structure represents the first example of a CRD determined from a galectin which
does not show the canonical 2-fold symmetric dimer organization. Comparison with
the published structures of galectins-1 and -2 provides an explanation for the
differences in carbohydrate-binding specificity shown by galectin-3, and for the
fact that it fails to form dimers by analogous CRD-CRD interactions.
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Figure 2.
Fig. 2. The human galectin-3 carbohydrate binding site.
Residues interacting with the bound LacNAc moiety through direct
and water mediated hydrogen bonds or through van der Waals
contacts are shown. The bound LacNAc moiety is shown with yellow
bonds. Oxygen and nitrogen atoms are colored red and blue,
respectively. Water molecules are labeled W1-W3. Potential
hydrogen bonds are shown as dotted lines.
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Figure 3.
Fig. 3. Electrostatic potential of the galectin-3-C
surface viewed into the carbohydrate binding site. The molecular
surface was generated using GRASP (31) with a probe radius of
1.4 Å. Blue and red indicate positive and negative
electrostatic potentials respectively. The bound LacNAc moiety
is shown in stick representation in yellow.
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The above figures are
reprinted
by permission from the ASBMB:
J Biol Chem
(1998,
273,
13047-13052)
copyright 1998.
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Secondary reference #1
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Title
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Structure of s-Lectin, A developmentally regulated vertebrate beta-Galactoside-Binding protein.
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Authors
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D.I.Liao,
G.Kapadia,
H.Ahmed,
G.R.Vasta,
O.Herzberg.
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Ref.
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Proc Natl Acad Sci U S A, 1994,
91,
1428-1432.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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X-Ray crystal structure of the human dimeric s-Lac lectin, L-14-Ii, In complex with lactose at 2.9-A resolution.
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Authors
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Y.D.Lobsanov,
M.A.Gitt,
H.Leffler,
S.H.Barondes,
J.M.Rini.
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Ref.
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J Biol Chem, 1993,
268,
27034-27038.
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PubMed id
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