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PDBsum entry 1a2v
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Amine oxidase
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PDB id
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1a2v
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystallographic study of yeast copper amine oxidase.
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Authors
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R.Li,
L.Chen,
D.Cai,
J.P.Klinman,
F.S.Mathews.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1997,
53,
364-370.
[DOI no: ]
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PubMed id
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Abstract
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The copper-containing amine oxidase from the yeast Hansenula polymorpha (YAO)
has been crystallized and partially solved by molecular replacement. It
catalyzes the oxidative deamination of primary amines by molecular oxygen to the
corresponding aldehydes, ammonia and hydrogen peroxide. It contains a covalently
bound redox cofactor, topa quinone, generated by post-translational modification
of a single tyrosine side chain. The crystals of YAO are orthorhombic, with
space-group symmetry P2(1)2(1)2(1) and unit-cell dimensions a = 138.8, b =
148.2, c = 234.0 A and diffract X-rays beyond 2.0 A resolution. Solution by
molecular replacement using the E. coli amine oxidase structure [Parsons,
Convery, Wilmot, Yadav, Blakeley, Corner, Philips, McPherson & Knowles
(1995). Structure, 3, 1171-1184] as a search model reveals that there are three
dimers in the asymmetric unit in a trigonal arrangement having 32 point-group
symmetry. The solution agrees well with the self-rotation function of YAO. The
non-crystallographic threefold axis lies parallel to a crystallographic twofold
screw axis and each dimer has twofold symmetry. Phases from the refined model
based on the molecular-replacement solution were used to solve one heavy-atom
derivative. Model building from the unbiased isomorphous replacement phases is
in progress.
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Figure 1.
Fig. 1. Crystals of yeast copper amine oxidase. There ae four polymorphs observed under the same crystallization conditions: (a) and (b are
monoclinic crystls and (c) and (d) are orthorhombic crystals.
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Figure 3.
Fig. 3. Schematic drawing of the crystallographic asymmetric unit
based on the molecularreplacement solution. The asymmetric unit
consists of a trigonal arrangement of dimers and has pointgroup
symmetry D3 (32), with the local threefold axis parallel to the
crystallographic a axis. The directions of the dyads in each dimer
(the monomers are ditinguished by thin and thick lines) are
indicated with respect to the crystallographic b and c axes.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1997,
53,
364-370)
copyright 1997.
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