 |
PDBsum entry 178d
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Crystal structure of a DNA duplex containing 8-Hydroxydeoxyguanine-Adenine base pairs.
|
|
|
Authors
|
|
K.E.Mcauley-Hecht,
G.A.Leonard,
N.J.Gibson,
J.B.Thomson,
W.P.Watson,
W.N.Hunter,
T.Brown.
|
|
|
Ref.
|
|
Biochemistry, 1994,
33,
10266-10270.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The crystal structure of the oligonucleotide d(CGCAAATTO8GGCG), containing the
chemically modified base 8-hydroxydeoxyguanine (O8G), has been determined at
2.5-A resolution and refined to a crystallographic R-factor of 16.8%. The B-type
DNA helix contains standard Watson-Crick base pairs except at the mismatch
sites, where O8G adopts a syn conformation and forms hydrogen bonds to adenine
in the anti conformation. The thermodynamic stability of the duplex was found by
UV melting techniques to be intermediate between the native oligonucleotide
d(CGCAAATTTGCG) and an oligonucleotide containing A.G mispairs d(CGCAAATTGGCG).
Comparison of the structure of the O8G(syn).A(anti) base pair with those of
Watson-Crick base pairs has given a reason why O8G.A base pairs are not well
repaired by DNA proofreading enzymes.
|
|
|
|
|
|
|
|
Headers
|
|
|
|
|
|
