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Figure 5.
Fig. 5. Stereo view of the mutated site of prolyl
oligopeptidase. A, D641A variant; the corresponding Asp641 side
chain is shown as the thin line. B, D641N variant. Residues
638-643 of the wild-type enzyme are shown as thin lines (from
PDB entry 1qfm). The hydrolyzed substrate suc-Gly-Pro-OH is
shown darker than the protein residues. The SIGMAA (50) weighted
2mF[o]   F[c]
electron density using phases from the final model is contoured
at 1  level,
where represents
the r.m.s. electron density for the unit cell. Contours more
than 1.4 Å from any of the displayed atoms have been
removed for clarity. Dashed lines indicate hydrogen bonds (drawn
with MolScript; see Refs. 51 and 52).
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