Figure 3.
Fig. 3. Mobility, surface properties, and shape. (A) C trace of
NOS[ox] 114 (cubic
crystal form) colored by the crystallographic^ temperature
factor (low to high B factors colored blue to red) and displayed
with heme and mutation sites that affect function. Mutation
sites (side chains displayed and labeled by residue number)
affecting dimerization, L-Arg binding, or H[4]B binding
(defined^ in Fig. 2) cluster to highly mobile (red) projecting
regions. The view is rotated by about 45° from Fig. 1 about
a vertical axis. (B) Solvent-accessible molecular surface of
flattened^ (left) and concave (center) face. The orientation is
the same^ as in (A). The exposed heme edge (gold), residues
contributing to the distal pocket (cyan), and exposed conserved
hydrophobic^ residues (green) (defined in Fig. 2) map to the
same flattened^ face of the molecule and cluster in the regions
of high mobility and mutational sensitivity shown in (A), making
this surface the^ prime candidate for a symmetric dimer
interface. (C) Solvent-accessible^ molecular surface of the
narrow curved face. This face has few conserved exposed
hydrophobic residues. The view is rotated 90° from (A) and
(B) around a vertical axis.
trace of
NOS[ox] 
114 (cubic
crystal form) colored by the crystallographic^ temperature
factor (low to high B factors colored blue to red) and displayed
with heme and mutation sites that affect function. Mutation
sites (side chains displayed and labeled by residue number)
affecting dimerization, L-Arg binding, or H[4]B binding
(defined^ in Fig. 2) cluster to highly mobile (red) projecting
regions. The view is rotated by about 45° from Fig. 1 about
a vertical axis. (B) Solvent-accessible molecular surface of
flattened^ (left) and concave (center) face. The orientation is
the same^ as in (A). The exposed heme edge (gold), residues
contributing to the distal pocket (cyan), and exposed conserved
hydrophobic^ residues (green) (defined in Fig. 2) map to the
same flattened^ face of the molecule and cluster in the regions
of high mobility and mutational sensitivity shown in (A), making
this surface the^ prime candidate for a symmetric dimer
interface. (C) Solvent-accessible^ molecular surface of the
narrow curved face. This face has few conserved exposed
hydrophobic residues. The view is rotated 90° from (A) and
(B) around a vertical axis.