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Figure 2.
Figure 2 The PIF-pocket. (A) A surface representation of the
putative PIF-binding pocket is shown and compared with the
pocket interacting with the C-terminal FXXF motif in PKA. For
PDK1, the  G-helix
of a symmetry-related molecule is shown as a purple coil, in PKA
the C-terminus is shown as a purple coil. Aromatic amino acids
buried in the pocket are shown as sticks with green carbons,
further side chains interacting with the pocket are shown with
orange carbons. Helix C
is shown as a green ribbon in both PDK1 and PKA. In PDK1, the
ordered sulfate ion and basic residues interacting with it are
also shown. The asterisk indicates residues from the symmetry
related molecule. (B) A stereo image of the residues lining the
PIF/ phosphate-pockets is shown. The PDK1 backbone is shown as a
grey ribbon. Side chains are shown with carbon atoms coloured
orange. Hydrogen bonds to the sulfate ion are shown as black
dotted lines.
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