Figure 2.
FIG. 2. GdnHCl-induced equilibrium unfolding of H.
thermophilus cytochrome c[552] (circles) and P. aeruginosa
cytochrome c[551] (squares) monitored by Trp fluorescence at pH
4.7, 10 °C. Lines are the best fit to a two-state model as
follows: [GdnHCl] = 4.0 ± 0.1 M
and 2.1 ± 0.1 M for H. thermophilus cytochrome c[552] and
P. aeruginosa cytochrome c[551], respectively; m-values 2.2
± 0.1 kcal mol-1 M-1 and 2.4 ± 0.1 kcal mol-1 M-1
for H. thermophilus cytochrome c[552] and P. aeruginosa
cytochrome c[551],respectively.
= 4.0 ± 0.1 M
and 2.1 ± 0.1 M for H. thermophilus cytochrome c[552] and
P. aeruginosa cytochrome c[551], respectively; m-values 2.2
± 0.1 kcal mol-1 M-1 and 2.4 ± 0.1 kcal mol-1 M-1
for H. thermophilus cytochrome c[552] and P. aeruginosa
cytochrome c[551],respectively.