Figure 1 - full size

Figure 1.
Figure 1: Superhelical path and base-pair-step parameters for NCP147 DNA. a, Structural alignment of the NCP147 (gold) and best-fit, ideal superhelix (red) paths. The NCP147 DNA structure is superimposed (gold). The left view is down the superhelix axis and the right view is rotated 90° around the pseudo-two-fold axis (vertical). b, Roll, tilt, shift, slide and twist base-pair-step parameters. The base-pair-step values plotted are the means for the two halves of the symmetrical sequence (shown above, with the dyad position labelled '0'). The iCAT and iSAT curves (red) show the roll and tilt contributions to the ideal superhelix curvature. The minor-groove blocks show base-pair-step shift alternation (pink in shift and tilt) or kinks (orange in roll, slide and twist). The primary bound-phosphate groups are indicated above the base sequence by pointers (numbered as the 5' phosphate of the adjacent base) showing the strand direction (dark, 3' arrow 5'; light, 5' arrow 3') and the interacting histone motif (L1, loop 1; L2, loop 2; A1, 1).