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Figure 3.
Figure 3: A ring of five trimers of E1*. a, b, Surface
representation of the ring, coloured according to domains, view
down the five-fold axis (a) and a side view (b) slightly tilted
to show the depth of the crater. Note the grey stem segment
leading to the C terminus of the fragment, indicated by arrows.
c, d, Micrographs of negatively stained rosettes of E1* trimers
obtained by dialysing away the detergent used for
solubilization. In the right-hand panel, a star was added to
highlight the trimers present in rings of five. The three-fold
symmetry of some of the trimers is evident, especially on panel
c. e, Model for a symmetric rosette exhibiting dodecahedral
symmetry, built according to Supplementary Information. The
magnification is about three times that of the rosettes
presented in panels c and d.
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