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Figure 3.
FIG. 3. Structure of BX-320 bound to the catalytic domain
of PDK1 (PDB code 1Z5M [PDB]
). We crystallized the PDK1 catalytic domain (residues 51-359)
as described by Biondi et al. (30). BX-320 was introduced into
PDK1 crystals and the structure determined from x-ray
diffraction data to a resolution 2.17 angstroms. As shown,
BX-320 binds in the ATP binding pocket, making two hydrogen
bonds through aminopyrimidine nitrogens to Ala^162 located in
the hinge region.
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