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Figure 3.
Fig. 3. A: PCR amplifications of the genomic region between
5′ and 3′ TT AOX1 sequences in six representative
transformants, MN1, MN2, MN3, MN4, MN5, MN6. Lane 6 shows the
amplification positive control, i.e. the 744 bp PCR product
(including the MUP coding segment) of the region limited by the
same AOX1 sequences in the pHIL-D2MUP vector. The MUP cDNA is
integrated in all tested Mut^s recombinants. The wild-type AOX1
gene (2.2 kb) is absent because replaced by the MUP expression
cassette. B: SDS-PAGE analysis indicating that rMUP is
accumulated in the expression culture of clone MN2 during an
induction cycle: 15 μl of medium, collected at the indicated
induction times, was loaded on the gel. Staining with Coomassie
blue detected rMUP only.
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