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Figure 1.
Figure 1. cDNA for X29 ProteinThe complete nucleotide
sequence for X29 is shown with a translation below. The
initiating methionine is indicated in bold. Underlined peptides
were obtained by Edman sequencing or MS/MS. The NUDIX consensus
sequence is boxed, with the adjacent glutamic acid residues
essential for catalysis indicated. The KK in the NUDIX domain
indicates the X29 EE→KK mutation (see text). Degenerate
oligonucleotides corresponding to peptides labeled #7 and #8
(dashed underline) yielded the 350 bp fragment by RT-PCR. X29
Genbank Accession Number, AY423379.
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