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Figure 7.
Figure 7. Zif23-GCN4( -2) fused to the VP16 activation
domain activates a promoter containing the -2 site in vivo. The
normalized luciferase activity for each combination of
expression and reporter vectors is indicated as a vertical bar.
Each value represents the average of three to five experiments
with the standard error of the mean indicated as a vertical line
near the top of each bar. (a) Normalized luciferase activity
observed from various reporters when transfected into 293 cells
with the Zif23-GCN4( -2)-VP16 expression vector. The identity of
the binding sites in each reporter is indicated below each
column. The pGL2 control refers to the parent reporter vector
without any binding sites inserted. (b) Normalized luciferase
activity observed from the -2 site reporter when transfected
into 293 cells with the various expression vectors. The identity
of each expression vector is indicated below each column. The
Rc-CMV2 control refers to the parent expression vector without a
gene inserted downstream of the CMV promoter.
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