Figure 5 - full size

Figure 5.
Fig. 5. Effect of overproduced MalE31 on DegP. Fractions from cellular fractionation experiment shown in Fig. 1A were separated by SDS-polyacrylamide gel electrophoresis, and immunoblot analysis of the gel was performed by using a rabbit antibody directed against DegP. A, whole cell extracts. B, periplasmic and membrane fractions. Lanes 1, pHCME31 in pop6590. Lanes 2, pHCME31 in NS2. Lanes 3, pHCME31 in pop6499. Lanes 4, p 709 in pop6499.