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Figure 5.
Figure 5. Staining of Splenocytes with Biotinylated Ly49A
EC(A) Splenocytes (1 × 10^6) from C57Bl/6 (H-2^b), BALB/c
(H-2^d), and C3H/HeJ (H-2^k) mice were incubated with
biotinylated Ly49A EC and PE-SA and analyzed by flow cytometry
as described in the Experimental Procedures. Background staining
with PE-SA alone was identical for all three strains. Staining
of splenocytes from BALB/c (B), C57BL/6 (C), and β2m^−/−
(D) mice was performed as above, using a lower concentration of
the bio-Ly49A (dark lines). Competition with unlabeled Ly49A
(dotted lines) was carried out (see the Experimental Procedures).
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