Figure 4 - full size

Figure 4. Figure 4. The acetyl-lysine substrate-binding site and trimer formation by yeast Hst2. (a) Detailed view of the intermolecular interactions between yHst2 and the N-terminal extended loop from a symmetry-related molecule (indicated with a prime designation). Residues that mediate interactions are cyan, the N-terminal extended loop is green and the 13 helix is yellow. (b) Superposition with the p53 acetyl-lysine substrate (red) extracted from the Af2 Sir2-p53 peptide complex. (c) 2F[o] - F[c] electron density map contoured at 1 showing the N-terminal loop comprising residues 1 -7 of yHst2. (d) Schematic representation of the trimer with the N-terminal extended loop and 13 helix in yellow. (e) Detailed view of the trimer interface. Symmetry-related residues are indicated with a prime designation.