Figure 3 - full size

Figure 3.
FIGURE 3. The reaction scheme of mP5N-1. A, mP5N-1 has two enzymatic activities: nucleotidase activity (steps 1 and 2a) and phosphotransferase activity (steps 1 and 2b). B, the proposed catalytic mechanism for nucleotidase activity of mP5N-1. "R" represents ribonucleoside. Individual states of the reaction mechanism include apoenzyme (I), active enzyme (II), the substrate complex (III), the substrate-transition complex (IV), the phosphoenzyme intermediate (V), the product-transition complex (VI), and the product complex (VII).