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Figure 2.
(a) Absorption spectrum of purified A. thaliana cry3. The
dominant absorption peak at 384 nm typical of MTHF absorption
and the shoulders at 450 and 480 nm typical of absorption of
two-electron oxidized form of FAD are clearly visible. Inset:
absorption spectra in the 500 --700 nm region shown at larger
resolution with two peaks (at 590 and 640 nm) typical of the
absorption of flavin neutral radical FADH . The concentration of
cry3 was 1.3 mg ml^[minus sign]1. (b) Fluorescence emission and
excitation (b) spectra of purified A. thaliana cry3. Emission
spectra are shown as solid lines and excitation spectra as
broken lines. Emission of excited flavin showing a peak at 520
nm and excitation spectra for this emission are shown in red.
Emission of excited MTHF* with a peak at 460 nm overlapping the
flavin emission peak and excitation spectra for emission at 460
nm are shown in blue. Inset: emission and excitation spectra in
the 300 --600 nm region shown at larger resolution. The
concentration of cry3 was 50 [micro]g ml^[minus sign]1. Acta
Crystallogr Sect F Struct Biol Cryst Commun. 2005 October 1;
61(Pt 10): 935–938. Published online 2005 September 30. doi:
10.1107/S1744309105028897. Copyright [copyright] International
Union of Crystallography 2005
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