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Figure 2.
Figure 2. Aminoacylation of RNA Substrates by
Aa-AlaRS453(A) The sequences of A. aeolicus tRNA^Ala and
synthetic RNA substrates tested for aminoacylation by
Aa-AlaRS453. Base pairs in the acceptor-stem region of
Ec-minihelix^Ala that differ from the A. aeolicus sequence are
labeled with asterisks.(B) Aminoacylation of wild-type
Aa-minihelix^Ala (closed circles) and G3:C70 Aa-minihelix^Ala
(open circles) by Aa-AlaRS453 at 37°C (pH 7.5). Inset: the
same experiment repeated at 55°C. Aminoacylation of
Ec-minihelix^Ala was done at 37°C only (closed triangles).
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