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Figure 1.
Figure 1. Alignments of amino acid sequences of ThkA and TrrA.
Their genomic sequences were completely analyzed (GenBank
accession no. AE000512).^15 Asterisk and plus symbols denote
conserved and similar residues, respectively, compared with FixL
(in (a)) and DivK (in (b)). (a) Alignment of ThkA (pir id:
H72262) and three other species of HK. The maximum matching for
Sinorhizobium melliloti FixL (131  vert,
similar , pir id: S39984), T. maritima HK-TM0853 (232 vert,
similar , pdb id: 2C2A) and E. coli PhoQ (335 vert,
similar , pdb id: 1IDO) to ThkA were analyzed by comparison
with Δ408, Δ517 and Δ591ThkA, respectively. The residues in
the conserved motifs (H, N, G1, F, and G2-box) of HK are
colored red. The proposed roles of each conserved motif are as
follows: H-box, containing phosphorylated His; N-box, divalent
cation (Mg^2+) binding site; G1, F and G2-box, ATP-binding
site.^7 Cys634 and Cys721 of ThkA are colored blue. The
ThkA-Cys721 position corresponds to PhoQ-Ala449, which is
adjacent to the ATP binding pocket revealed by the crystal
structure.^5 (b) Alignment of TrrA (pir id: A72263) and
Caulobacter crescentus DivK (pdb id: 1M5T). Met55 of TrrA is
colored magenta. Figure 1. Alignments of amino acid sequences
of ThkA and TrrA. Their genomic sequences were completely
analyzed (GenBank accession no. AE000512).[3]^15 Asterisk and
plus symbols denote conserved and similar residues,
respectively, compared with FixL (in (a)) and DivK (in (b)). (a)
Alignment of ThkA (pir id: H72262) and three other species of
HK. The maximum matching for Sinorhizobium melliloti FixL (131
[4]not, vert, similar , pir id: S39984), T. maritima HK-TM0853
(232 [5]not, vert, similar , pdb id: 2C2A) and E. coli PhoQ (335
[6]not, vert, similar , pdb id: 1IDO) to ThkA were analyzed by
comparison with Δ408, Δ517 and Δ591ThkA, respectively. The
residues in the conserved motifs (H, N, G1, F, and G2-box) of HK
are colored red. The proposed roles of each conserved motif are
as follows: H-box, containing phosphorylated His; N-box,
divalent cation (Mg^2+) binding site; G1, F and G2-box,
ATP-binding site.[7]^7 Cys634 and Cys721 of ThkA are colored
blue. The ThkA-Cys721 position corresponds to PhoQ-Ala449, which
is adjacent to the ATP binding pocket revealed by the crystal
structure.[8]^5 (b) Alignment of TrrA (pir id: A72263) and
Caulobacter crescentus DivK (pdb id: 1M5T). Met55 of TrrA is
colored magenta.
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