Figure 1.
Fig. 1. Amino acid sequences of Y/S monobodies. (A) A
schematic drawing of the monobody scaffold. -Strands A–G and the
three loops that are diversified in the library are indicated.
(B) Affinity and amino acid sequences of Y/S monobodies that
were selected from the initial library selection. The number of
occurrences for clones that appeared more than once is indicated
in parentheses. K[d] values determined by using yeast surface
display are also shown. The sequences for the three loops are
shown, with the numbering of Main et al. (20). Tyr, Ser, and the
other amino acids are shaded in yellow, red, and gray,
respectively.
-Strands A–G and the
three loops that are diversified in the library are indicated.
(B) Affinity and amino acid sequences of Y/S monobodies that
were selected from the initial library selection. The number of
occurrences for clones that appeared more than once is indicated
in parentheses. K[d] values determined by using yeast surface
display are also shown. The sequences for the three loops are
shown, with the numbering of Main et al. (20). Tyr, Ser, and the
other amino acids are shaded in yellow, red, and gray,
respectively.