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Figure 1.
Fig. 1. Expression and purification of the Orf17 protein. A
polyacrylamide gel (15%) containing 1% SDS, stained with
Coomassie Blue, contained the following: lane 1, 4 µg each
of bovine serum albumin (66 kDa), ovalbumin (45 kDa), carbonic
anhydrase (31 kDa), and MutT (15 kDa); lanes 2 and 3, 1 µl
of crude extract from cells transformed with pET11b (no insert)
or pETorf17, respectively; lanes 3-5, approximately 4 µg
of Fractions I, II, or III, respectively (see ``Methods '').
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