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Figure 1.
(a) Polyacrylamide gel electrophoresis under denaturing
conditions of purified A. thaliana cry3. Lane 1, Sigma
molecular-weight markers 7B (kDa; Sigma-Aldrich, St Louis, MO,
USA). Lane 2, purified cry3 (10 [micro]g) used for
crystallization. The gel was stained with Coomassie blue. (b)
Typical morphology of A. thaliana cry3 crystals. Crystals were
obtained by equilibration against a solution of 85 mM sodium
citrate pH 4.6, 170 mM ammonium acetate, 21.5% PEG 4000 and 7.5%
glycerol. The average dimensions of the cry3 crystals were about
0.2 x 0.1 x 0.1 mm. (c) Self-rotation function calculated with a
native A. thaliana cry3 data set employing data between 20.0 and
2.5 A resolution. A self-rotation search with the program MOLREP
at the angle [chi] = 180[deg] was used to identify the twofold
rotation axis. Acta Crystallogr Sect F Struct Biol Cryst Commun.
2005 October 1; 61(Pt 10): 935–938. Published online 2005
September 30. doi: 10.1107/S1744309105028897. Copyright
[copyright] International Union of Crystallography 2005
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