Figure 1 - full size

Figure 1. Fig. 1. PAL gene from yeast R. toruloides was cloned in the expression vector pIBX-7 where transcription is controlled by the strong inducible tac promoter and terminated by the rRNA transcription terminator sequences rrnBT1 and rrnBT2. LacI^q represses the tac promoter, and hence isopropyl -D-thiogalactoside is required to release it from the promoter. The kanamycin resistance gene (Kan^R) is included in the construct to allow selection of cells containing the plasmid.