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Figure 2.
Figure 2. Anti-Ly49A Monoclonal Antibodies Specifically
Bind to Ly49A EC and Ly49A NKD-LPurified preparations of the
three indicated molecules were immobilized to a CM-5 chip and
analyzed for binding to the indicated mAbs by SPR using the
BIACore 2000 as described in the Experimental Procedures.
Solution phase analytes (mAbs) were injected at T = 83 s.
Dissociation washout was initiated at T = 365 s. To evaluate the
potential for deterioration of the coupled surfaces with
successive cycles of binding and regeneration, mAb A1 was used
in the first (solid circle) and last (open square) runs.
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