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Figure 1.
Fig. 1. Fractionation of soluble and insoluble MalE
variants. Cells grown in rich medium containing 0.2% maltose at
30 °C for 3 h were fractionated from spheroplasts.
Periplasmic (soluble) and membrane fractions (insoluble),
corresponding to 10^8 bacteria, were separated by
SDS-polyacrylamide gel electrophoresis (12.5% acrylamide). The
gels were stained with Coomassie Blue or silver-stained. A,
strains harboring pHCME31 or p  709
plasmid. B, strains harboring pHCME-I33P or p 709 plasmid.
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