|
Figure 1.
Fig. 1. First reversed-phase HPLC separation of immune
hemolymph of H. virescens. The 40% acetonitrile fraction
obtained after prepurification by solid phase extraction was
analyzed on an Aquapore RP-300 C [8] column. Elution was
performed with a linear gradient (dotted line) of acetonitrile
in acidified water. Absorbance was monitored at 225 nm (solid
line). Antimicrobial activities were detected by liquid growth
inhibition assays against M. luteus, (white column), E. coli
(dotted column), and N. crassa (black column). The inset shows
the final purification of fraction A, which contains the active
antifungal compound.
|
