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Figure 1.
Fig. 1. Sequence alignment of MsrAs and proteins
containing putative MsrA domains. The longer N-terminal
sequences preceding the region of strong homology are not shown.
Filled squares mark those sequences that have been truncated at
the C terminus. The locations of the cysteine residues of bMsrA
are indicated by filled circles. The alignment was generated by
using the CLUSTAL W algorithm in MACVECTOR 6.0 (Oxford Molecular
Group). Dark and light background shading illustrate regions
having greater than 51% identity or similarity, respectively.
Spaces added for insertions are shown by dashed lines. The
sequence of Neisseria gonorrhoeae (NEIGO) MsrA was redetermined
(see text) and the region corresponding to Cys-72 in bMsrA was
found to be G205GCFWGLEAYFQRIDGVVDAVSG227, which differs from
that determined previously (A^205AASGAWGAWKPISNASTAWLTRYR226)
(22). The remainder of the sequence within the MsrA domain was
unchanged. Abbreviations: ARATH, Arabidopsis thaliana (accession
number, P54150); BACSU, Bacillus subtilis (P54154); BOVIN, Bos
taurus (P54149); BRANA, Brassica napus (P54151); DROME,
Drosophila melanogaster (P08761); ERWCH, Erwinia chrysanthemi
(AJ012716); FRAAN, Fragaria ananassa (P54152); GRACI, Gracilaria
gracilis (AAD43253); HAEIN, Haemophilus influenzae (P45213);
ECOLI, Escherichia coli (P27110); HELPY, Helicobacter pylori
(O25011); HUMAN, Homo sapiens (CAB59628); LYCES, Lycopersicon
esculentum (P54153); MYCGE, Mycoplasma genitalium (P47648);
MYCPN, Mycoplasma pneumoniae (P75188); MYTB, Mycobacterium
tuberculosis (CAB07043); NEIGO, Neisseria gonorrhoeae (P14930);
SCHIPO, Schizosaccharomyces pombe (Q09859); STRPN, Streptococcus
pneumoniae (P35593); YEAST, Saccharomyces cerevisiae (P40029).
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