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Figure 1.
Fig. 1. Absorbance decay of the semiquinones at 450 nm as
a function of time after the second of two laser flashes in the
presence of various concentrations of ZnSO[4] (a) and CdSO[4]
(b). From the decay, the rate constant k[AB]^(2) was determined.
Note the slowing of the kinetics with increasing cation
concentrations. The pedestal at long times after the laser flash
is caused by the absorbance change of the cytochrome c used to
reduce the primary donor (see Materials and Methods). Conditions
were: 2 µM RCs in 10 mM Tris·HCl (pH 7.7), 0.25%
lauryl dimethylamine-N-oxide with the concentration of ZnSO[4]
or CdSO[4] as indicated in the figure.
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