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Figure 9.
Figure 9. Proposed reaction mechanism of CKS. The b- and
g-phosphates of CTP are bound in the so-called PP-loop. Kdo is
displayed at the position of the NeuAc moiety of bound CMP-NeuAc
without trying to state a contact to a surrounding residue.
During the nucleophilic attack Arg10 moves from the b-phosphate
away to Asp51, and Lys19 moves from the b- to the a-phosphate. A
strongly bound water molecule between the carboxylates of Asp98
and Asp225 is considered to be the base accepting the hydroxyl
proton.
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