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Figure 8.
FIGURE 8. Stereoviews of the superimposition of the
catalytic sites of the ternary structures of Cr-P4H-1,
HIF-P4H-2, and CAS. A, stereo view showing the active site of
the ternary structure of Cr-P4H-1 superimposed on that of
HIF-P4H-2 complexed with inhibitor and Fe^2+ (PDB code 2G19). B,
superimposition of the ternary Cr-P4H-1 active site with the CAS
active site complexed with Fe^2+ and 2-oxoglutarate in the
presence or absence of NO. The Cr-P4H-1 is shown with gray
carbons and bonds in both panels. In panel A HIF-P4H-2 and the
bound inhibitor are in cyan. In panel B CAS is shown in yellow,
whereas the two modes of binding of 2-oxoglutarate are in yellow
(in the complex without NO; PDB code 1DRY) and magenta (in the
complex with NO, PDB code 1GVG). The water molecules in the
active sites of HIF-P4H-2 (two waters) and CAS (one water in the
structure with NO) are shown in magenta.
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