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Figure 7.
Fig. 7. Binding of ssDNA by the TraI-CT measured by
fluorescence anisotropy. TraI 1476–1756 at 75 and 150 mM NaCl
is indicated by continuous and dashed blue lines (K[d] = 2.9 μM
and K[d] = 7.7 μM), respectively. TraI 1476–1756 with a
deletion of the proline-rich loop at 75 and 150 mM NaCl is
indicated by continuous and dashed red lines (K[d] > 17.1 μM
and K[d] > 15.9 μM), respectively. TraI 1476–1756 with
mutations of prolines 1518, 1523, and 1525 to glycine at 75 and
150 mM NaCl is indicated by continuous and dashed green lines
(K[d] > 13.9 μM and K[d] > 18.2 μM), respectively, while the
binding of 1476–1630 at 150 mM NaCl is indicated in black
(K[d] > 22.6 μM).
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