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Figure 7.
Fig. 7. Identification and localization of conserved residues
in SufI. (a) CueO: the conserved residues Gly113 and Gly114
(green sticks) sit above the trinuclear copper centre (shown as
transparent spheres). Residues Gly117, Arg125 and Val127 (blue
sticks) form the brim of a surface cavity that exposes the
112–114 loop (DGG in CueO, conserved as DGX across all
multicopper oxidases) to the solvent. (b) SufI: in green, the
glycine residues Gly114 and Gly115 corresponding to the glycines
in (a); in blue, the residues Arg118, Trp126 and Pro128, which
are all highly conserved across SufI sequences and prevent
solvent access to the 112–114 loop in the SufI structure. (c)
A surface representation of SufI, coloured red (surface residue
most conserved) through orange, yellow, and green, to blue
(least conserved). The N- and C-termini of the protein are shown
for reference and highly conserved surface residues are
labelled. A black star marks the location of the residues
118–128 that cover the pocket corresponding to the CueO
catalytic centre. The right-hand panel shows a second view of
the SufI surface, rotated with respect to the left-hand panel by
180° around the vertical axis.
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