Figure 7.
Figure 7. Maturase-RNA interactions. (a) Site-directed
cleavage patterns superimposed on a three-dimensional model for
the bI3 maturase and P5-P4-P6 domain complex. Spheres, sites of
derivatization; colored RNA backbones, cleavage sites; green,
-strands
of the bI3 maturase. (b) Summary of 2'-O-methyl interference in
the P5b and P5c helices. Maturase is shown slightly transparent
for clarity. Red spheres, sites of 2'-O-methyl interference
(Supplementary Fig. 1); blue backbone, solvent-based hydroxyl
radical cleavage sites (Fig. 5c,d).
-strands
of the bI3 maturase. (b) Summary of 2'-O-methyl interference in
the P5b and P5c helices. Maturase is shown slightly transparent
for clarity. Red spheres, sites of 2'-O-methyl interference
(Supplementary Fig. 1); blue backbone, solvent-based hydroxyl
radical cleavage sites (Fig. 5c,d).