Figure 7 - full size

Figure 7.
FIG. 7. Schematic of xylan hydrolysis by the xylan degrading enzymes of C. japonicus. The structures shown for CjXyn11A and CjXyn11B are representative bacterial GH11 enzymes from Bacillus agaradhaerens and Nonomuraea flexuosa, respectively, the structure shown for CjAbf51A is from Geobacillus stearothermophilus, and the structure shown for CjXyn10D is of the homologous (93% similar) enzyme CmXyn10B from C. mixtus. The enzymes CjAbf51A, CjGlcA67A, and CjXyn10C are all attached to the outer membrane of the bacterium, whereas CjXyn10A, CjXyn11A, and CjXyn11B are secreted into the culture medium (40, 42, 43). The sugar side chains released by the -glucuronidase and arabinofuranosidase, respectively, are likely to be transported into the cytoplasm of C. japonicus. (The depiction of the plant cell wall is reproduced from Carpita and Gieaut (44). Reproduced with permission from Blackwell Publishing, ©1993.)