Figure 6.
FIGURE 6. Structural comparison of Atg3 with canonical E2s
bound to their targets. A, ribbon diagram of Atg3. Color coding
is as described in the legend to Fig. 1B. The side chain of
Cys-234 and a sulfate ion observed near Cys-234 are shown with
stick models. B, ribbon diagram of Ubc12 bound to the Ubl domain
of UBA3, a subunit of the E1 for NEDD8 (Protein Data Bank code
1Y8X). Conserved -helices and -strands
in Atg3 and Ubc12 are colored red and cyan, respectively. The
side chain of Cys-111 is shown with a stick model. C, ribbon
diagram of SUMO-RanGAP1 conjugate bound to Ubc9 (Protein Data
Bank code 1Z5S). Although Nup358, an E3 enzyme, is also bound to
this complex, the protein is not shown in this figure. Gly-97 of
SUMO and the side chains of Ubc9 Cys-93 and RanGAP1 Lys-524 are
shown with stick models. The figure was prepared using PyMOL.
Conserved -helices and -strands
in Atg3 and Ubc9 are colored red and cyan, respectively.
-helices and 
-strands
in Atg3 and Ubc12 are colored red and cyan, respectively. The
side chain of Cys-111 is shown with a stick model. C, ribbon
diagram of SUMO-RanGAP1 conjugate bound to Ubc9 (Protein Data
Bank code 1Z5S). Although Nup358, an E3 enzyme, is also bound to
this complex, the protein is not shown in this figure. Gly-97 of
SUMO and the side chains of Ubc9 Cys-93 and RanGAP1 Lys-524 are
shown with stick models. The figure was prepared using PyMOL.
Conserved